AlgX is a periplasmic protein required for the production of the exopolysaccharide alginate in Pseudomonas sp. and Azotobacter vinelandii. AlgX has been overexpressed and purified and diffraction-quality crystals have been grown using iterative seeding and the hanging-drop vapor-diffusion method. The crystals grew as flat plates with unit-cell parameters a=46.4, b=120.6, c=86.9Å, β=95.7°. The cyrstals exhibited the symmetry of space group P21 and diffracted to a minimimum d-spacing of 2.1Å. On the basis of the Matthews coefficient (VM=2.25Å3 Da-1), two molecules were estimated to be present in the asymmetric unit.
Weadge, Joel T.; Yip, Patrick P.; Robinson, Howard; Arnett, Krista; Tipton, Peter A.; and Howell, P. Lynne, "Expression, Purification, Crystallization and Preliminary X-Ray Analysis of Pseudomonas aeuginosa AlgX" (2010). Biology Faculty Publications. 43.