Document Type

Article

Publication Date

1976

Department

Biology

Abstract

Isolation of the four major peroxidase isozymes (isozymes 1, 2, 3, and 4) of two flax genotypes was achieved by modifying the procedure used by Shannon et al. (1966) for the isolation of horseradish peroxidase isozymes. The net positive and net negative charges of isozymes 1, 2, and 4 were different. Isozyme 3 resembled isozyme 4 in charge but differed in apparent molecular weight. The chromatographic elution profiles of both genotypes were the same. Anionic gel electrophoresis demonstrated that after isolation and repurification, relative mobility differences existed between the corresponding isozymes of the two genotypes for all four isozymes.

Comments

This article was originally published in Canadian Journal of Botany, 54(11): 1180-1188. © 1976 National Research Council. Reproduced with permission

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