Reproducible Hairy Root Transformation and Spot-Inoculation Methods to Study Root Symbioses of Pea

Authors

Scott R. Clemow, Wilfrid Laurier UniversityFollow
Lindsey Clairmont, Wilfrid Laurier UniversityFollow
Lene H. Madsen, Aarhus University
Frédérique C. Guinel, Wilfrid Laurier UniversityFollow

Document Type

Article

Publication Date

12-15-2011

Department

Biology

Abstract

Pea has lagged behind other model legumes in the molecular study of nodulation and mycorrhizae-formation because of the difficulty to transform its roots and its poor growth on agar plates. Here we describe for pea 1) a transformation technique which permits the complementation of two known non-nodulating pea mutants, 2) a rhizobial inoculation method which allows the study of early cellular events giving rise to nodule primordia, and 3) a targeted fungal inoculation method which allows us to study short segments of mycorrhizal roots assured to be infected. These tools are certain to advance our knowledge of pea root symbioses.

Comments

This article was originally published in Plant Methods, 7(46). It is distributed under the Creative Commons Attribution License.

Recommended Citation

Clemow, Scott R.; Clairmont, Lindsey; Madsen, Lene H.; and Guinel, Frédérique C., "Reproducible Hairy Root Transformation and Spot-Inoculation Methods to Study Root Symbioses of Pea" (2011). Biology Faculty Publications. 6.
https://scholars.wlu.ca/biol_faculty/6