Peroxidase isoenzynles may be separated on acrylamide gels and then detected by supplying the substrate in an appropriate reaction system. One such system frequently used contains guaiacol as the hydrogen donor, although this compound has certain drawbacks. Ways of circumventing these drawbacks are suggested, so that quantitative estimates of the activity of individual peroxidase isoenzymes may be obtained.
Fieldes, Mary Ann; Tyson, H.; and Bloomberg, R., "Measurement of Activity of Peroxidase Isoenzymes in Flax (Linum usitatissimum)" (1971). Biology Faculty Publications. Paper 16.